DFG-Forschergruppe "Regional Climate Change": Microbial regulation of organic matter decomposition at the regional scale

Our project provides the analytical basis for improving the parameterisation of biogeochemical models (e.g., DAISY-EXPERTN) by introducing dynamic feedbacks between belowground communities and climate. Whereas we have measured seasonal and regional variation of temperature sensitivity functions of soil microbial processes involved in carbon cycling during the first phase of the project, we will now focus on group specific turnover times (bacteria and fungi) and flow of carbon into specific groups of soil microorganisms and their seasonal substrate use efficiency.

A microcosm experiment will be established in cropped plots at both regions (Swabian Alb and Kraichgau) to study the turnover times of bacterial and fungal biomass using the ¹³C-PLFA and ¹³C-ergosterol profiling approach. We hypothesise that fungi store newly assimilated carbon (¹³C signal) for a longer period than bacteria due to their slower turnover times. Manipulating the input of resources in a field experiment will allow us to investigate separately the C flow through bacteria and fungi. We will choose the following labelled compounds: (1) ¹³C glucose (to account for mainly bacterial C incorporation) and (2) ¹³C phenol/lignin precursors (to account for mainly fungal C incorporation). We will establish the microcosms four times a year (early spring, summer, autumn and late autumn). Microbial efficiency will be assessed by measuring ¹³C-substrate incorporation into different components of the microbial biomass (C_mic, bacterial and fungal PLFAs, ergosterol) and loss as ¹³CO₂ in the field for up to 48 hours after installation of the microcosms. Our results will be used by project partners to examine the sensitivity of multi-compartment carbon models (e.g. CN DAISY) to altered microbial growth efficiency. In addition, we will test which of the following: substrate quality, site specific microbial decomposer communities, or abiotic soil properties, is the dominant driver of spatial variation in litter decomposition at the regional scale.

Subproject P9 of the DFG Research Unit 1695 "Agricultural Landscapes under Global Climate Change – Processes and Feedbacks on a Regional Scale"